Why measure sample viscosity?

In most biophysical methods, a change in viscosity will impact the readout. Thus, to get correct readouts, you have to identify if there is a change in viscosity and compensate for the change. With hydrodynamic radius (Rh), for instance, an increase of viscosity can lead to over-estimation of Rh and vice versa. The Fida 1 automatically detects viscosity changes and compensates for such.

Fida 1 measures sample viscosity with every run and enables automatic viscosity correction of Rh, without any requirement of manual post-processing of the data.

‍The correction for viscosity change is automatically built into every FIDA measurement (incl. binding studies, stability studies, and quantifications). In other words, you do not need to correct anything manually, and the data is correct even if there are unexpected viscosity changes.

In every FIDA measurement, the viscosity is calculated based on the time lapse from sample introduction in the capillary till it reaches the detector (the peak point). Thereby, the Fida 1 can also be used as a very accurate viscosity meter capable of measuring viscosities from 0.89 cP up to +20 cP with an ability to measure changes as low as 0.2 cP. On top of that, in Fida 1 instrument, temperature-induced viscosity changes are managed thanks to its built-in temperature control.

Read about viscosity measurement in Biophysical Analysis, Drug Formulation and Bioprocessing here.

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