How to work with sticky samples?

In FIDA it is immediately clear, when stickiness reaches a level which might disturb the data readout. Stickiness is not causing problems in FIDA. Moderate sticky material can be run with regularFIDA capillaries. With stronger stickiness, you may apply a dedicated, dynamic coating to regular capillaries or permanently coated capillaries. This can be purchased on  shop.fidabio.com (registered users only).

Detection of indicator adsorption

Assays performed at low nano-molar protein concentrations are often impacted by sample loss due to passive adsorption to glass/plasticware, ultimately leading to a reduction in fluorescence signal. FIDA is a great tool to detect this tendency of molecules and thus enables the user to take appropriate measures to prevent it. Below is a suggested method that can be used to spot adsorption of the indicator:

Note: Reagents like pluronic acid, Tween-20 and BSA act as surfactants and aid in preventing sample adsorption to the surface. These reagents may also diminish the tailing effect of FIDA taylorgrams (discussed in paragraph 1.2).

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