Approximate Hydrodynamic Radius - Molecular Weight Calculator
What is hydrodynamic radius (Rh)?
Hydrodynamic radius (Rh), is a parameter used to describe the size of a particle or molecule in a fluid (solution), taking into account its shape, mass, and interactions with the surrounding fluid molecules. The hydrodynamic radius is defined as the radius of a hypothetical sphere that diffuses at the same rate as the particle or molecule in question, under the same conditions (
Einstein, 1905
). In-solution techniques such as flow-induced dispersion analysis (FIDA) are used to determine the hydrodynamic radius experimentally.
What is molecular weight?
- Molecular weight (mass) is the sum of the atomic weights of all atoms in a molecule, represented with the unit kDa. In general, larger molecules tend to have higher molecular masses than smaller molecules, but the relationship between molecular weight and size is not always straightforward.
- The conversion from hydrodynamic radius to molecular weight is done by running globular proteins of known mass and plotting the retention time versus the molecular weight on a double logarithmic plot as a calibration curve. Note that this method assumes that all proteins are globular, which is not always the case.
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