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Absolute Concentration Data:
Use direct quantification – free from reliance on reference standards or curves, ensuring more reliable data.
fida
FIDA Quantification:
Fast Titer Determination Directly in Complex Matrices
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Absolute quantification – for any sample.
FIDA technology delivers precise, absolute quantification of molecular species in solution, requiring only minimal sample volumes (from microliters to nanoliters). With FIDA, you can quantify with confidence, even in unpurified or complex samples.
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What difference does FIDA Quantification make?
Work in Complex Samples:
Quantify molecules even in unpurified samples, such as e.g. fermentation media or patient samples, without the need for extensive sample prep.
Low Sample Consumption:
Use only nanoliters to microliters of sample for quantification, conserving valuable materials.
Increased Sensitivity:
Have the capacity of detecting and quantifying even low concentrations - ideal for early-stage discovery or low-abundance targets.
Robust for Diverse Sample Types:
From proteins to nanoparticles, FIDA delivers consistent quantification across a wide range of sample types, making it a versatile tool for various fields.
Get Multiple Readouts in One Assay:
FIDA allows for simultaneous quantification and characterization (such as binding affinity and kinetics), saving your time and increasing ‘sample size-to-data obtained’ ratio.
What is the practial impact?
In comparison to other quantification methods (e.g. ELISA), FIDA stands out for its absolute measurement capabilities and sample versatility. ELISA, for instance, in addition to result uncertainty, often requires extensive assay development, calibration and is limited by antibody availability and specificity. Similarly, methods like mass spectrometry and HPLC can require lengthy preparation and purification steps, as well as more sample volume. FIDA’s in-solution approach minimizes these steps, providing faster, more efficient quantification that works even in unpurified samples. Additionally, FIDA’s ability to measure low concentrations without loss of accuracy makes it ideal for scenarios where sensitivity is critical, e.g. during work with low-abundance targets.
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