Quality Control - Detecting Labelling Issues Early to minimise loses:
FIDA helps quality control teams detect labelling errors that might affect a proteins size or behaviour. Identifying these issues early ensures optimal assay performance
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Labeling Quality
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Instant feedback on labeling efficiency and sample integrity
FIDA’s automatic labeling quality measurement is embedded in every assay and measurement, providing instant feedback on labeling efficiency and sample integrity. This ensures that researchers can trust their results without needing additional steps for quality control. By detecting potential issues with labeling early, FIDA helps maintain experiment reliability, minimizes rework, and increases overall productivity—saving both time and resources.
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What is labeling quality parameter used for?
Experimental Research - Ensuring Functional Data:
In research, accurate labelling is key to reliable results. FIDA ensures that labels do not introduce artifacts or alter the sample’s natural behaviour, allowing researchers to refine labelling strategies for consistent, high-quality data.
How is labelling quality assessed?
Rh measurements can assess the impact of labelling on molecular structure. FIDA’s labelling quality measurement detects changes in the hydrodynamic radius (Rh) that might indicate improper or excessive labelling. Poor or excessive labelling (e.g., with fluorophores or biotin) can alter the molecule’s conformation, increasing its Rh. FIDA can monitor these changes, ensuring that the labelling process has not significantly altered the molecule’s natural size or behaviour, thereby confirming the quality of labelled samples. Further, Fida measures any free label not conjugated to the protein.
Optimising drug functionality in biopharmaceuticals:
Labelling quality metric helps ensuring that labelling does not affect drug functionality. If labelling alters the size or conformation of the biopharmaceutical, it can affect its functionality or stability. This feedback allows researchers to fine-tune the labelling process, ensuring minimizing the risk of misinterpreting results.
FAQs
Would you like to know more? See frequently asked questions below. If you do not find an answer to your question, you can ask a question to one of our scientists.
What do we understand by labelling quality?
At FIDA, with ‘labeling quality’ we usually mean free label, it is to say non conjugated fluorephore. Please note that in the areas of protein production or protein quality control we typically do not label the protein prior to analysis. Here we use intrinsic (UV) fluorescence.
Can Fida Instruments work with noncovalent labeling?
Yes, there are several non-covalent labels that will work examples that has been applied in the past are: - HIS Lite™ OG488-Tris NTA-Ni Complex against the His-tag. - Single domain antibodies against the ALFA-tag. - As well as nanobodies specific for your protein of interest.
Could over-labeling your molecule have a negative effect on FIDA measurement?
No, over-labeling will have no impact on the sizing measurement using Fida. However, mind that for all technologies that rely on labelling to detect interactions over-labelling risks putting a label in or nearby the binding site inhibiting the binding due to stearic hindrance.
What labelling strategies are there?
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