Protein expression

Big insights from small samples
I'm Interested

How does FIDA accelerate protein expression?

01

Save sample material thanks to pre-purification characterisation:

  • Down to 4 μL analyte, 40 nL indicator
  • Purified and non-purified, with no buffer constraints
  • Functional characterisation in non-purified samples prevents sample waste
  • 8 simultaneous and embedded quality control parameters in every measurement improve assay quality
02

Simultaneous validation of protein functionality and integrity

  • Simultaneous expression and function assessment based on quantity (expression level), affinity measurement (Kd) and protein integrity (Rh)
  • One technology for various workflow stages
03

Short assays

  • No need for purification
  • Sample quality control in 4 minutes
  • 8 simultaneous QC readouts
  • Temperature controlled autosampler (2x50 vials and 96 well plates) saves your time
04

Embedded quality control

  • Absolute size
  • Polydispersity index (PDI)
  • Quantification of aggregates
  • Sample loss
  • Stickiness
  • Viscosity
  • PDB correlator
  • Labelling quality

Simultaneous expression and function assessment

FIDA users benefit from monitoring and quantification of the protein of interest at various stages during the protein expression process, allowing for rapid process optimisation and quality control.

Increases your productivity

The FIDA technology is applicable for biomolecular quantification and for measuring binding affinity directly in complex samples, which reduces sample purification requirements and your assay development time. This can increase users’ productivity and success rate.

Protein Expression Timeline

Gene Synthesis & Cloning

Transformation of host cells

Screening and selection

Expression optimisation

FIDA characterisation in unpurified samples allows for optimisation of expression conditions (temperature, pH, media composition), allowing FIDA users to make informed process adjustments and increase the yield, quality and solubility of their protein.

Clone selection

One FIDA readout delivers both quantity and affinity measurements, providing a sound base for clone selection and minimising the sample and waste created by purifying underperforming clones.

Cell Harvesting & Lysis

FIDA detects changes in the concentration of the protein as the cell lysate is passed through the FIDA instrument. Thus, it can be used to monitor the efficiency of cell lysis and protein release during the cell harvesting and lysis step.

Protein Purification

FIDA can be used to monitor the efficiency of protein purification, as it delivers information on polydispersity, aggregates and affinity.

Protein Characterisation

With 8 built-in simultaneous QC parameters, FIDA is the go-to protein characterisation tool.

Scale Up

FIDA has an intuitively simple interface, making it an approachable tool even for non-lab workers or students. For industrial scaling, read more about biopressing

Storage

FIDA’s size measurement can track degradation that might occur over time.

Clone selection

Clone screening based on both clone performance and protein quality

Deconvolution of the Kd and expression levels.

Ability to run assays in unpurified media

Ability to perform clone selection performed before purification.

Absolute measure of Rh in nanometres, reveals the fraction of your protein of interest that is bound.

The hydrodynamic radius serves as a QC check of the proteins, since degradation results in changes in size and/or loss of binding.

Targeting common Protein Expression Challenges

Challenge
Protein folding and stability
FIDA is used to monitor protein folding and stability. Measurement of changes in the hydrodynamic radius of a protein over time helps researchers to optimise protein expression conditions and identify chaperones or other factors that can improve protein folding and stability.
Difficulties in scale-up
Yield, quality and bulletproof assay design are necessary to successfully scale up protein expression. FIDA delivers information on protein folding, stability and protein-protein interactions, which combined with its straightforward assay design makes it a perfect tool for protein expression scaling.

This methodology is very sensitive and useful for assessing binding in a quantitative manner using small amounts of auto-fluorescent proteins. Using this method, we can observe clear changes in apparent hydrodynamic radius (Rh) and in fluorescence signal of EYA1 as a function of increasing concentrations of peptides or of small molecules

Dana Farber Institute
CANCER INSTITUTE

''Fida  Instrument provides a ''ruler'' to measure the length of fibrils''

Stefan Rüdiger Ph.D.
Professor of Protein Chemistry of Disease; Head of Department of Chemistry

''The FIDA is very effective for screening small molecules for solubility and interactions with protein targets. A major advantage has been its ability to clearly show drug binding to difficult-to-work-with targets such as intrinsically disordered proteins and amyloids''

Dr. Alexander Taylor
Research Fellow in Biochemistry/Biophysics

I would say that this instrument is quite useful for core facilities at KI or for Swedish research community in general:

  1. it was possible to measure unlabeled membrane proteins and soluble proteins at low concentrations in small volumes very fast (~6 minutes per measurement).
  2. I also like the temperature control and the autosampler which allowed us to run a lot of samples overnight.
  3. I would say that there is a lot of potential to perform other assays as well, such as the Kd determinations etc. (For this, I use ITC which is quite time-consuming and requires a lot of protein).
  4. In addition, the software and user interface for sample runs and data analysis is quite straightforward and intuitive to use.
Postdoctoral Researcher

"What we value about FIDA is the amount of information we can get from a tiny amount of protein in a single QC run. FIDA technology enables us to understand our proteins better - particularly when they behave in unexpected ways. We chose FIDA because it gives us that vital first result quickly - we don't waste our time optimising an assay that isn't going to work."

Duncan Smith
Lead Scientist

"I am convinced. I have never before been able to detect the ternary construct formation of my construct directly in cell lysate – it took less than two hours."

Thomas Smith
Associate Director, Chemical Biology & Therapeutics

"The Fida 1’s unique capability for measuring binding Kd from weak mM level to strong pM level really enhances our bioanalytical capabilities."

Sherry Zhu
Senior Scientist

"FIDA is an in-solution technique, and thus an ideal binding assay for structurally diverse bsAbs because the analysis does not rely on potentially obstructive surface immobilization and hence it is able to perform characterizations that are unbiased by bsAb format and spatial orientation of the binding domains."

Andreas V. Madsen
PhD Student

"After thorough comparisons, it was clear that the Fida 1, amongst others, presents unique opportunities in analysis of membrane proteins, which is essential to us."

Svend Kjæer
STP Deputy

''The Fida 1 system offers a new approach to detergent screening that has significant advantages allowing essential data to be obtained faster using less material (…) this work presents a new approach to characterize membrane proteins that allows users to reducing costs and time as well as to analyze protein expressed at low levels in a short time thus overcoming any stability issues.''

Isabel Moraes
Principal Research Scientist

"I like the ease of use and the straightforward data analysis and, that experiments can be performed in any type of buffers. We get data, where no other biophysical methods worked before."

Cathrine Birck
Head of Integrated Structural Biology Platform

"The method is easy to setup in any standard laboratory and can be adopted for a high throughput (HTTP) screening, which enabled mechanistic studies of RNA nuclease interactions, as well as efficient guide RNA lead discovery (…) Fida 1 offers straightforward assay development, walk away automation, absolute measurement and ultra low sample consumption."

Denny Truong
Principal Research Associate

"FIDA provides in-depth assessment of activity combined with local and global protein structural changes by measuring the overall hydrodynamic radius of the protein with minimal sample consumption. In addition, it is possible to measure the affinity constant for the analyzed interaction."

Dr. Melanie Hug
Research Associate

"The beauty of Fida 1 is that it allows us to conduct a lot of biophysical measurements in one system using a small amount of reagent and a rapid read-out time which is important for our projects and to our customers. (…) Providing a high-quality, protein characterisation QC package is certainly extending and future proofing our capabilities in biophysical characterisation of the proteins we produce."

Mark Abbott
Managing Director

"The Fida 1 has allowed us to assess the performance of MIPs with a much higher throughput than was previously possible on other platforms. MIPs can be developed in as little as 8 weeks, which is a significant improvement on antibodies, and now they can be fully characterized at an accelerated pace too."

Alan Thomson
CEO

"Fida 1 can be used for full characterization of ternary complex formation for targeted protein degradation. The Fida 1 platform only consumes 39 nL of sample for one measurement and thus allows elaborate condition screening using very small amounts of sample material."

Roman Agafonov
Associate Director of Biochemistry, Biophysics & Structural Biology

"The Fida1 is a very user-friendly system. With the Fida software we can quickly design new methods, set up experiments, and analyze data. By far this is a great instrument and technology that has allowed us to run binding assays for LNP in solution and with flexibility to run in any complex media which we were unable to do using traditional binding methods that required ligand immobilization. The FidaBio customer service is amazing, they are very knowledgeable and responsive whenever I run into issues and need assistance. I highly recommend this instrument for LNP or any other nanoparticle characterization. The system and technology are versatile with applications beyond nanoparticles and is a must in the biophysical tool kit."

Biophysical Analytical Department
Senior Scientist, Analytical Development

"Eventually, with FIDA, we managed to characterise our LNPs. And it was done in less than 2 days. We had for a long time been struggling with SPR."

Chemical Biology and Therapeutics
Associate Director

"We had been looking for ways of quantifying our exosomes, and tested many platform, without success. In less than 2 days FIDA had developed an assay and shown trustworthy quantifications directly in fermentation broth"

Rane Harrison
Director, Analytical Development

Become a user

Your laboratory instruments should serve you, not the other way around. We’re happy to help you.