Knowledge Base
Technology
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Sample
Becoming and being a FIDA user
Protein analysis and characterisation
Consumables
Protein stability and storage

How does Fida 1 detect a signal?

FIDA requires a fluorescent signal coming from your sample that can be detected by one of our detectors. It can either be emitting from a label or from the molecule itself, i.e. label-free. The signal coming from your sample that can be detected by one of our detectors: 480, 550, 640, 280.

FIDA uses a LED excitation and amplifies  the emission signal by PMT. Therefore, FIDA does not use any laser to get a signal, and the sample is not heated. Note that FIDA delivers two orthogonal readouts: absolute size (hydrodynamic radius) and fluorescence.