Assay design enables in-silico assay development and protocol generation. All the relevant parameters can be documented, processed, and evaluated upfront ahead of wet lab experiments. The assay design tool helps the user to find the methods specifically for its sample for optimal data generation. For ease of use, users can generate protocols in .doc format containing all defined information as well as detailed dilution series for sample preparation. Assay specific methods and sequences can be exported directly to the Fida 1 instrument, which saves time and minimises risks of human error.