Proteins and particles are sensitive structures. Their functions are often impacted by environmental changes, by labelling or by immobilization to a surface structure and functionality of the protein. All-in-all, in-solution studies increase data reliability, due to their ability to mimic the natural physiological conditions in which proteins and particles function. In solution approach allows to better understand the dynamic molecular interactions, conformational changes, and biochemical reactions, leading to more accurate insights into their behavior and enabling the development of more targeted and effective applications in various fields, including drug discovery, diagnostics, and biotechnology.