Is it needed to centrifugate your sample prior to FIDA measurement?

No, in general, it is not mandatory to centrifuge purified samples prior to FIDA measurements. However, if data retrieved by FIDA runs indicates presence of large aggregates (seen as signal spikes), samples can be centrifuged before performing binding assays. Moreover, centrifugation of sample might be necessary when working with crude cell lysates, to get rid of large fragments or cell debris. FIDA Quality Control, such as quantified aggregation measurement can be useful to determine this need.