Stickiness

In FIDA it is immediately clear, when stickiness reaches a level which might disturb the data readout. Stickiness is not causing problems in FIDA. Moderate sticky material can be run with regular FIDA capillaries. With stronger stickiness, you may apply a dedicated, dynamic coating to regular capillaries or permanently coated capillaries. This can be purchased on shop.fidabio.com (registered users only)

Fidabio data analysis software can tolerate mild stickiness without impacting the readout. In cases of strong stickiness between the sample and the capillary there are a few methods that can be used. It’s important to note that the capillary wall is made of fused silica, hence it will have an induced negative charge. This can cause interactions with proteins. To overcome this, you can: 1. Coat the capillary with our dynamic coating solution 2. Use our permanently coated capillary. 3. Modify the buffer with e.g. salt or change the pH to limit the interaction between the proteins and the capillary wall.

Fidabio data analysis software can tolerate mild stickiness without impacting the readout. In cases of strong stickiness between the sample and the capillary, a permamently coated capillary or a reversible surface coating can be can be used. For more on stickiness read the article For capillary or coating purchases visit shop.fidabio.com (registered users only)

When a protein is sticking to the capillary walls it will change the readout. We typically observe stickiness on the readout as a skewed gaussian signal. The software will indicate the stickiness issue, so you will be able to solve it.

Yes, Fida Software can indicate if the sample is sticking to the capillary walls. it can also indicate whether the issue os after assay optimisation.

In a Fida 1 experiment a sticky sample is when it is interacting with the fused silica that is the capillary wall.

Stickiness is an unspecific interaction between a molecule and e.g. a surface. The stickiness of biomolecules is influenced by various factors, including the presence of specific binding sites, electrostatic interactions, van der Waals forces, and hydrophobic interactions. Knowing the stickiness of your sample, enables you to ensure that you are actually measuring the molecule or the complex, you want to analyse and avoid misinterpretations of your data. Read more here.