Aggregation

Aggregation is usually measured as a function of a given parameter (time, different buffers, temperature etc.) all of which can be assayed with FIDA. The emergence of the different types of aggregates can then by quantified by measuring the number of spikes (insoluble aggregates) and the total fluorescent area of larger species (soluble aggregates).

Different types of aggregates can be observed with FIDA: soluble diffusive aggregates and insoluble non-diffusive aggregates. Soluble aggregates will register as a shoulder or bump on the Taylor-gram, while insoluble aggregates will register as a signal spike. The soluble kind can be quantified by their total fluorescent area by fitting multiple species. The insoluble ones can be counted by counting the spikes, as each spike is at least one aggregate, press to see the example.

Reasons for protein sample aggregation can vary. Press to see the answer.

Aggregation in molecular biology refers to the degradation process by which biomolecules, such as proteins or nucleic acids, cluster together, often forming larger complexes. After synthesis, proteins typically fold into a particular three-dimensional conformation (native state). This folding process can be impaired, creating protein misfolding or unfolding and lead to the protein aggregate. Keep reading...