In this work, it is shown that Flow Induced Dispersion Analysis (FIDA) is used to measure the unfolding of Human Serum Albumin (HSA) and the loss of bind- ing affinity to Fluorescein. The assay is performed under native conditions with minimal sample consumption. FIDA is a new capillary-based technology for measuring binding affinity and assessing protein stability in-solution under native conditions. FIDA utilizes Taylor dispersion for accurate size determinations of analytes in a pressure driven flow system. The change in apparent size forms the basis for an accurate measure of binding affinity and protein stability.

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