Protein stability and functionality Application Note

In this work, it is shown that Flow Induced Dispersion Analysis (FIDA) is used to measure the unfolding of Human Serum Albumin (HSA) and the loss of bind- ing affinity to Fluorescein. The assay is performed under native conditions with minimal sample consumption. FIDA is a new capillary-based technology for measuring binding affinity and assessing protein stability in-solution under native conditions. FIDA utilizes Taylor dispersion for accurate size determinations of analytes in a pressure driven flow system. The change in apparent size forms the basis for an accurate measure of binding affinity and protein stability.

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