pipettes

Rapid, Precise Information for Fast and Safe Drug Development

Based on our revolutionary, patented FIDA technology, Fidabio offers rapid, precise information on complex binding interactions and concentration of proteins, particles up to 1,000 nm Dh, antibodies, and other biomolecules.

The core measurements of the FIDA technology, or Flow-Induced-Dispersion- Analysis, are very precise and reproducible, absolute detections of hydrodynamic radii directly in plasma, serum, fermentation media, cell lysate etc. with accuracies of +/- 5 %, coefficient of variations of <5 % and a sensitivity of 0.1 nM FITC type tags. The instrument is fully automated for vials or 2 x 96 wells plates with end-to-end temperature control from 4-55 °C controllable in increments of 0.1 °C. Each test only requires a few hundred nanoliters of sample material.

The FIDA technology provides significant advantages over current technologies in terms of data quality, cost, versatility and time.

In other words, Fidabio enables scientists and researchers to carry out tests that are impossible using current technologies leading to faster and safer drug development.

Fida 1 Overview

Membrane Proteins & Nanoparticles

Multispecific Constructs

Application Notes

Publications

2022
Jack Wade, Charlotte Rimbault, Hanif Ali, Line Ledsgaard, Esperanza Rivera-de-Torre, Maher Abou Hachem, Kim Boddum, Nadia Mirza, Markus-Frederik Bohn, Siri A. Sakya, Fulgencio Ruso-Julve, Jan Terje Andersen, & Andreas H. Laustsen:
Generation of Multivalent Nanobody-Based Proteins with Improved Neutralization of Long α-Neurotoxins from Elapid Snakes
Bioconjugate Chem. 2022, 33, 8, 1494–1504
Link

2022
Andreas V. Madsen, Oscar Mejias-Gomez, Lasse E. Pedersen, Kerstin Skovgaard, Peter Kristensen, Steffen Goletz: Immobilization-Free Binding and Affinity Characterization of Higher Order Bispecific Antibody Complexes Using Size-Based Microfluidics 
Anal. Chem. 2022, 94, 40, 13652–13658
Link


Complicated Quantifications

Application notes

Publications

2022 
Morten E. Pedersen, Jesper Østergaard, Bente Glintborg, Merete L. Hetland & Henrik Jensen: Assessment of immunogenicity and drug activity in patient sera by flow-induced dispersion analysis 
Nature Scientific Reports 12, 4670 (2022) 
Link 

2020 
Morten E. Pedersen, Jesper Østergaard, and Henrik Jensen: In-Solution IgG Titer Determination in Fermentation Broth Using Affibodies and Flow-Induced Dispersion Analysis.
ACS Omega 2020, 5, 18, 10519–10524 
Link 

2019 
Pedersen, M. E.; Østergaard, J.; Jensen, H.  
Flow-Induced Dispersion Analysis (FIDA) for Protein Quantification and Characterization.  
In Methods in Molecular Biology; 2019. 
Link 

2015 
Poulsen, N. N.; Andersen, N. Z.; Østergaard, J.; Zhuang, G.; Petersen, N. J.; Jensen, H.  
Flow Induced Dispersion Analysis Rapidly Quantifies Proteins in Human Plasma Samples.  
Analyst 2015140 (13), 4365–4369.  
Link 

Complex Interactions

Application notes

Publications

2022 
Morten E. Pedersen, Jesper Østergaard & Henrik Jensen: Quantification of Structural Integrity and Stability Using Nanograms of Protein by Flow-Induced Dispersion Analysis
Molecules 2022, 27(8), 2506 
Link 

2022 
Julio Vacacela, Anna-Lisa Schaap-Johansen, Patricia Manikova, Paolo Marcatili, Marta Prado, Yi Sun, Jon Ashley: The Protein-Templated Synthesis of Enzyme-Generated Aptamers
Angewandte Chemie International Edition, 2022, e202201061 
Link 

2022
Nikolaj Riis Christensen, Christian Parsbæk Pedersen, Vita Sereikaite, Jannik Nedergaard Pedersen, Maria Vistrup-Parry, Andreas Toft Sørensen, Daniel Otzen, Lise Arleth, Kaare Teilum, Kenneth L. Madsen and Kristian Strømgaard: Bi-directional protein-protein interactions control liquid-liquid phase separation of PSD-95 and its interaction partners
iScience 2022, 25, 2, 103808
Link 

2021 
Emil G. P. Stender, Soumik Ray, Rasmus K. Norrild, Jacob Aunstrup Larsen, Daniel Petersen, Azad Farzadfard, Céline Galvagnion, Henrik Jensen & Alexander K. Buell: Capillary flow experiments for thermodynamic and kinetic characterization of protein liquid-liquid phase separation 
Nat Commun 12, 7289 (2021) 
Link 

2021 
Julián Valero, Laia Civit, Daniel M. Dupont, Denis Selnihhin, Line S. Reinert,  Manja Idorn, Brett A. Israels, Aleksandra M. Bednarz,  Claus Bus,  Benedikt Asbach, David Peterhoff,  Finn S. Pedersen, Victoria Birkedal, Ralf Wagner, Søren R. Paludan, & Jørgen Kjems: A serum-stable RNA aptamer specific for SARS-CoV-2 neutralizes viral entry 
PNAS December 14, 2021 118 (50) e2112942118 
Link 

2021 
Daniel E. Otzen, Alexander K. Buell, Henrik Jensen: Microfluidics and the quantification of biomolecular interactions
Current Opinion in Structural Biology, 2021, 70, 8-15  
Link 

2021 
Adrian Krzyzanowski, Raphael Gasper, Hélène Adihou, Peter ‘t Hart and Herbert Waldmann: Biochemical Investigation of the Interaction of pICln, RioK1 and COPR5 with the PRMT5-MEP50 Complex 
ChemBioChem 10.1002/cbic.202100079 
Link 

2021 
Morten E. Pedersen, Ragna M.S. Haegebaert, Jesper Østergaardb, and Henrik Jensen: Size-based characterization of adalimumab and TNF-α interactions using Flow Induced Dispersion Analysis: Assessment of Avidity-stabilized Multiple Bound Species
Scientific reports 
Link 

2019 
Pedersen, M. E.; Gad, S. I.; Østergaard, J.; Jensen, H.: Protein Characterization in 3D: Size, Folding, and Functional Assessment in a Unified Approach
Anal. Chem. 2019. 
Link 

2019 
M. S. Restan, M. E. Pedersen, H. Jensen, and S. Pedersen-Bjergaard : Electromembrane Extraction of Unconjugated Fluorescein Isothiocyanate from Solutions of Labeled Proteins Prior to Flow Induced Dispersion Analysis 
Anal. Chem., 2019. 
Link 

2016 
Poulsen, N. N.; Pedersen, M. E.; Østergaard, J.; Petersen, N. J.; Nielsen, C. T.; Heegaard, N. H. H.; Jensen, H.: Flow-Induced Dispersion Analysis for Probing Anti-DsDNA Antibody Binding Heterogeneity in Systemic Lupus Erythematosus Patients: Toward a New Approach for Diagnosis and Patient Stratification
Anal. Chem. 201688 (18), 9056–9061. 
Link 

2010 
Jensen, H.; Østergaard, J.: Flow Induced Dispersion Analysis Quantifies Noncovalent Interactions in Nanoliter Samples.  
J. Am. Chem. Soc. 2010, 132 (12), 4070-4071. 
Link 

Applications Examples
Protein Integrity
Confirmation of Oligomeric State

Binding Affinity

Analysis of Large Particles

The FIDA technology allows the analysis of proteins and particles 0.5 to 1,000 nm in diameter. This enables analysis of exosomes, liposomes, vaccines, viral vectors, membrane-embedded proteins, intrinsically disordered proteins et. al.

With the Fidabio instrument you can amongst others analyse interactions between targets and membrane proteins and vaccines, characterization of exosomes, liposome and IDPs, quantification of specific exosomes in plasma, quantification of viral vectors in fermentation media etc.

Analysis Directly in Complex Matrices

It is well-known that detection in a buffer, sample preparations and immobilization from time to time impact the data reliability. Detection directly under native conditions ensures you that your decisions are based on the most biologically accurate data.

The FIDA technology is based on in-solution measurements. You can analyze directly in plasma, serum, cell lysate, fermentation media, CHO cells et al. Background noise is eliminated by an automatic adjustment of the baseline and the risk of adsorption is management with our advanced, proprietary capillary coating.

In-solution Validation of Immobilization Data

SPR and BLI are industry standards and are widely used for amongst others affinity Kd determination. Though often robust and reliable, occasionally the surface immobilization might result in difficult-to-interpret artefacts due to mass transport limitations.

With easy setup and validation of new assays and the consumption of only a few hundred nanoliters of sample material, the Fidabio instrument offers a possibility of fast and accurate orthogonal validation of Kd data generated with SPR, BLI etc.