Literature
Rapid, Precise Information for Fast and Safe Drug Development
Based on our revolutionary, patented FIDA technology, Fidabio offers rapid, precise information on complex binding interactions and concentration of proteins, particles up to 1,000 nm Dh, antibodies, and other biomolecules.
The core measurements of the FIDA technology, or Flow-Induced-Dispersion- Analysis, are very precise and reproducible, absolute detections of hydrodynamic radii directly in plasma, serum, fermentation media, cell lysate etc. with accuracies of +/- 5 %, coefficient of variations of <5 % and a sensitivity of 0.1 nM FITC type tags. The instrument is fully automated for vials or 2 x 96 wells plates with end-to-end temperature control from 4-55 °C controllable in increments of 0.1 °C. Each test only requires a few hundred nanoliters of sample material.
The FIDA technology provides significant advantages over current technologies in terms of data quality, cost, versatility and time.
In other words, Fidabio enables scientists and researchers to carry out tests that are impossible using current technologies leading to faster and safer drug development.
Fida 1 Overview
Fida 1 Fundamentals: Assessment of Sample Quality with every measurement
Poster: Assessment of sample quality with every measurement – Fida 1 Fundamentals
Technote: The Relationship between hydrodynamic radius and molecular weight
Membrane Proteins & Nanoparticles
Application notes
- Membrane protein detergent screening
- GPCR in Nanodisc
- Liposome – Peptide binding Characterization
- AAV Characterization
- PDB vs In-solution Correlation
Publications
2020
Cholak E, Bugge K, Khondker A, et al.: Avidity within the N-terminal anchor drives α-synuclein membrane interaction and insertion.
The FASEB Journal. 2020;00:1–21
Link
Posters
- High-Throughput Detergent Screening for Membrane Protein solubilization using nanoliters of sample
- Size-based characterization of peptide-liposome interactions
- 360-Degree Automated Characterization of AAVs – The Safe Solution for Gene Therapy
- PDB Correlator: Linking Structure and Function
White papers
Infographics
Multispecific Constructs
Application Notes
- In-solution Ubiquitination Assay
- Ternary Complex Characterization of TPD
- Bi-specifics, Ternary and Binary binding characterization
- PDB vs In-solution Correlation
- Agglutination of Multivalent Antibodies
Publications
2022
Jack Wade, Charlotte Rimbault, Hanif Ali, Line Ledsgaard, Esperanza Rivera-de-Torre, Maher Abou Hachem, Kim Boddum, Nadia Mirza, Markus-Frederik Bohn, Siri A. Sakya, Fulgencio Ruso-Julve, Jan Terje Andersen, & Andreas H. Laustsen:
Generation of Multivalent Nanobody-Based Proteins with Improved Neutralization of Long α-Neurotoxins from Elapid Snakes
Bioconjugate Chem. 2022, 33, 8, 1494–1504
Link
2022
Andreas V. Madsen, Oscar Mejias-Gomez, Lasse E. Pedersen, Kerstin Skovgaard, Peter Kristensen, Steffen Goletz: Immobilization-Free Binding and Affinity Characterization of Higher Order Bispecific Antibody Complexes Using Size-Based Microfluidics
Anal. Chem. 2022, 94, 40, 13652–13658
Link
Posters
- Characterization of Molecular Glues – an automated in-solution platform
- Rapid Sizing and Characterisation of Bispecific Antibodies
- Fully automated characterization of ternary complex formation
- PDB Correlator: Linking Structure and Function
- Using FIDA to measure gRNA-nuclease interactions for CRISPR-Cas development
Infographics
Complicated Quantifications
Application notes
- Fidabio Clone Selection Assay: Protein expression level and affinity directly in fermentation media without purification
- Selective quantification of IgG directly in F12 fermentation media
- Detection of auto-antibodies in Systemic Lupus Erythematosus patients
Publications
2023
Helena Ø. Rasmussen, Amit Kumar, Ben Shin, Fisentzos Stylianou, Lee Sewell, Yingqi Xu, Daniel E. Otzen, Jan Skov Pedersen, Steve J. Matthews,
FapA is an Intrinsically Disordered Chaperone for Pseudomonas Functional Amyloid FapC,
Journal of Molecular Biology,
Volume 435, Issue 2,2023, 167878
Link
2023
Ferapontov, A., Omer, M., Baudrexel, I. et al. Antigen footprint governs activation of the B cell receptor. Nat Commun 14, 976 (2023).
Link
2023
Ray, Soumik & Mason, Thomas & Boyens-Thiele, Lars & Jahnke, Nadin & Buell, Alexander. (2022). Mass photometric detection and quantification of nanoscale α-synuclein phase separation. 10.1101/2022.05.03.490467
Link
2022
Morten E. Pedersen, Jesper Østergaard, Bente Glintborg, Merete L. Hetland & Henrik Jensen: Assessment of immunogenicity and drug activity in patient sera by flow-induced dispersion analysis
Nature Scientific Reports 12, 4670 (2022)
Link
2020
Morten E. Pedersen, Jesper Østergaard, and Henrik Jensen: In-Solution IgG Titer Determination in Fermentation Broth Using Affibodies and Flow-Induced Dispersion Analysis.
ACS Omega 2020, 5, 18, 10519–10524
Link
2019
Pedersen, M. E.; Østergaard, J.; Jensen, H.
Flow-Induced Dispersion Analysis (FIDA) for Protein Quantification and Characterization.
In Methods in Molecular Biology; 2019.
Link
2015
Poulsen, N. N.; Andersen, N. Z.; Østergaard, J.; Zhuang, G.; Petersen, N. J.; Jensen, H.
Flow Induced Dispersion Analysis Rapidly Quantifies Proteins in Human Plasma Samples.
Analyst 2015, 140 (13), 4365–4369.
Link
Complex Interactions
Application notes
- Protein Oligomerization: Easy and Accurate Determination in Plasma and Buffer
- Correlating supramolecular behavior in buffer and plasma
- Characterization of Conformational Change
- Assessment of protein stability and functionality by Flow Induced Dispersion Analysis
- Liquid-Liquid Phase Separation (LLPS)
- Screening conformational stability using nanograms of protein
- Fidabio PDB Correlator
- Quantification and characterization of exosomes
Publications
2022
Morten E. Pedersen, Jesper Østergaard & Henrik Jensen: Quantification of Structural Integrity and Stability Using Nanograms of Protein by Flow-Induced Dispersion Analysis
Molecules 2022, 27(8), 2506
Link
2022
Julio Vacacela, Anna-Lisa Schaap-Johansen, Patricia Manikova, Paolo Marcatili, Marta Prado, Yi Sun, Jon Ashley: The Protein-Templated Synthesis of Enzyme-Generated Aptamers
Angewandte Chemie International Edition, 2022, e202201061
Link
2022
Nikolaj Riis Christensen, Christian Parsbæk Pedersen, Vita Sereikaite, Jannik Nedergaard Pedersen, Maria Vistrup-Parry, Andreas Toft Sørensen, Daniel Otzen, Lise Arleth, Kaare Teilum, Kenneth L. Madsen and Kristian Strømgaard: Bi-directional protein-protein interactions control liquid-liquid phase separation of PSD-95 and its interaction partners
iScience 2022, 25, 2, 103808
Link
2021
Emil G. P. Stender, Soumik Ray, Rasmus K. Norrild, Jacob Aunstrup Larsen, Daniel Petersen, Azad Farzadfard, Céline Galvagnion, Henrik Jensen & Alexander K. Buell: Capillary flow experiments for thermodynamic and kinetic characterization of protein liquid-liquid phase separation
Nat Commun 12, 7289 (2021)
Link
2021
Julián Valero, Laia Civit, Daniel M. Dupont, Denis Selnihhin, Line S. Reinert, Manja Idorn, Brett A. Israels, Aleksandra M. Bednarz, Claus Bus, Benedikt Asbach, David Peterhoff, Finn S. Pedersen, Victoria Birkedal, Ralf Wagner, Søren R. Paludan, & Jørgen Kjems: A serum-stable RNA aptamer specific for SARS-CoV-2 neutralizes viral entry
PNAS December 14, 2021 118 (50) e2112942118
Link
2021
Daniel E. Otzen, Alexander K. Buell, Henrik Jensen: Microfluidics and the quantification of biomolecular interactions
Current Opinion in Structural Biology, 2021, 70, 8-15
Link
2021
Adrian Krzyzanowski, Raphael Gasper, Hélène Adihou, Peter ‘t Hart and Herbert Waldmann: Biochemical Investigation of the Interaction of pICln, RioK1 and COPR5 with the PRMT5-MEP50 Complex
ChemBioChem 10.1002/cbic.202100079
Link
2021
Morten E. Pedersen, Ragna M.S. Haegebaert, Jesper Østergaardb, and Henrik Jensen: Size-based characterization of adalimumab and TNF-α interactions using Flow Induced Dispersion Analysis: Assessment of Avidity-stabilized Multiple Bound Species
Scientific reports
Link
2019
Pedersen, M. E.; Gad, S. I.; Østergaard, J.; Jensen, H.: Protein Characterization in 3D: Size, Folding, and Functional Assessment in a Unified Approach.
Anal. Chem. 2019.
Link
2019
M. S. Restan, M. E. Pedersen, H. Jensen, and S. Pedersen-Bjergaard : Electromembrane Extraction of Unconjugated Fluorescein Isothiocyanate from Solutions of Labeled Proteins Prior to Flow Induced Dispersion Analysis
Anal. Chem., 2019.
Link
2016
Poulsen, N. N.; Pedersen, M. E.; Østergaard, J.; Petersen, N. J.; Nielsen, C. T.; Heegaard, N. H. H.; Jensen, H.: Flow-Induced Dispersion Analysis for Probing Anti-DsDNA Antibody Binding Heterogeneity in Systemic Lupus Erythematosus Patients: Toward a New Approach for Diagnosis and Patient Stratification.
Anal. Chem. 2016, 88 (18), 9056–9061.
Link
2010
Jensen, H.; Østergaard, J.: Flow Induced Dispersion Analysis Quantifies Noncovalent Interactions in Nanoliter Samples.
J. Am. Chem. Soc. 2010, 132 (12), 4070-4071.
Link
Posters
- Probing non-Covalent Interactions and Quantifying of large Biomolecules
- PDB Correlator: Linking Structure and Function
- FIDA, DSF, BLI, DLS orthogonal quad data for Biophysical characterization of higher order protein interactions and stability analysis (poster by KEMP PROTEINS)
- Rapid Characterization of Liquid-Liquid Phase Separation with FIDA
White papers
Infographics
Analysis of Large Particles
The FIDA technology allows the analysis of proteins and particles 0.5 to 1,000 nm in diameter. This enables analysis of exosomes, liposomes, vaccines, viral vectors, membrane-embedded proteins, intrinsically disordered proteins et. al.
With the Fidabio instrument you can amongst others analyse interactions between targets and membrane proteins and vaccines, characterization of exosomes, liposome and IDPs, quantification of specific exosomes in plasma, quantification of viral vectors in fermentation media etc.
Analysis Directly in Complex Matrices
It is well-known that detection in a buffer, sample preparations and immobilization from time to time impact the data reliability. Detection directly under native conditions ensures you that your decisions are based on the most biologically accurate data.
The FIDA technology is based on in-solution measurements. You can analyze directly in plasma, serum, cell lysate, fermentation media, CHO cells et al. Background noise is eliminated by an automatic adjustment of the baseline and the risk of adsorption is managed with our advanced, proprietary capillary coating.
In-solution Validation of Immobilization Data
SPR and BLI are industry standards and are widely used for amongst others affinity Kd determination. Though often robust and reliable, occasionally the surface immobilization might result in difficult-to-interpret artefacts due to mass transport limitations.
With easy setup and validation of new assays and the consumption of only a few hundred nanoliters of sample material, the Fidabio instrument offers a possibility of fast and accurate orthogonal validation of Kd data generated with SPR, BLI etc.
