50x faster screening with FIDA (in-solution kinetics)

Tozaro (Previously MIP Discovery / Diagnostics) ingeniously reimagined its affinity reagent screening workflow using FIDA, transforming interaction analysis from slow and labor intensive to fast and precise. The benefit? They managed to achieve greater efficiency, scalability, and decision-making power in molecular interaction analysis.

What was the problem & the solution?

Traditional techniques such as Surface Plasmon Resonance (SPR) created throughput bottlenecks, limiting the ability to evaluate large polymer libraries quickly and cost-effectively. The solution: FIDA-Driven Workflow:

After these steps, only selected candidates are progressed to SPR to assess surface immobilization effects, eliminating the need for SPR in primary screening. By their innovative adaptation of FIDA, Tozaro replaced SPR for most polymer interaction screening analyses, streamlining research and accelerating discovery.

What were the benefits?

(1) 50x increase in throughput from ~6 polymers/day (SPR) to up to 288/day (FIDA).

(2) Only 30 min for small molecules to 3 hours for proteins long screens for a full 96-well plate screened in with FIDA. Meanwhile, SPR could process only ~6 samples/day, requiring 16+ days to screen the same number of samples.

(3) Up to 10x reduction in sample consumption – fewer replicates and lower volumes needed due to direct in-solution measurements.

(4) Only 1.5–2 hours per sample to get a binding affinity data (for an 8-point titration)

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By replacing SPR with FIDA for polymer interaction analysis, Tozaro streamlined research, reduced time-to-decision, and dramatically increased efficiency in polymer screening workflows.

We are proud to support such innovative scientists. If you'd like to know more about FIDA's in-solution kinetics:

‍watch our on-demand webinar

read our most recent publication on the topic

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