Quantification and Characterization of Exosomes

The present application provides an insight into characterisation of exosomes in terms of size, quantity, and affinity, by using Flow Induced Dispersion Analysis (FIDA). FIDA is a capillary-based technology for measuring binding affinity and complex size of biomolecules under native conditions (2,3,4). In the present project, an anti CD81 antibody and an anti-exosome antibody were used to probe binding affinities of exosomes in assay buffer and spent medium. Cholesterol-TopFluor was used as an indicator for sizing of exosomes.

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