High throughput detergent screening for membrane proteins using nanoliters of sample

We demonstrate how the Fida 1 is employed for high throughput detergent screening to assess the degree of solubilization and polydispersity of a GFP- tagged membrane protein. The screening takes place directly in crude matrix, using only 1,5 uL for an entire screen of 12 detergents. We compare the FIDA detergent screening with Fluorescence Size- Exclusion Chromatography (FSEC). The comparison reveals that Fida 1 decreases the screening time from days to hours and leaves most of your membrane protein available for other experiments.

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