Screening Protein stability
In this work, we demonstrate how the Fida 1 can be utilized for probing conformational stability of the therapeutic antibody adalimumab using as little as 39 ng per measurement. We report Cₘ- values and the free energy of unfolding for adalimumab at different pH values. The Fida 1 is ideal for assessing stability during drug development or when having scarce amounts of sample available.
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