IgG quantification in fermentation media Application Note

FIDA is a new capillary-based technology for quantifying biomolecular concentration directly in complex solutions (e.g. plasma) and for measuring in-solution binding under native conditions. FIDA is based on Taylor dispersion in a pressure-driven flow of a ligand (termed indicator, e.g. Protein-A) interacting with the analyte of interest (e.g. an IgG antibody). The indicator alone appears small (i.e. it has a small hydrodynamic radius) when it is not bound to the antibody, but upon binding it will appear larger (i.e. the complex has a larger hydrodynamic radius). The change in apparent size forms the basis for an accurate measure of analyte concentration and interaction. 

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