Systemic Lupus Erythematosus (SLE) is an autoimmune disease, where the detection of circulating auto-antibodies against ds-DNA is used as an important diagnosis criterion. However, current methods for detecting anti-ds-DNA antibodies in blood samples are primarily based on ELISA procedures utilizing surface chemistries (i.e. immobilization of antigen), multiple steps and cumbersome optimization. Here we present a simple and immobilization-free procedure, termed Flow-Induced Dispersion Analysis (FIDA), for detecting auto-antibodies against ds-DNA directly in 85 % plasma samples.

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